ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Molecular Devices SpectraMax M5e Microplate Reader. Donor: 620 nm. These isotypes cause antigen cross-linking by multimeric binding and self. MCE has not independently confirmed the accuracy of these methods. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. The cell survival. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. StakMax Microplate Handling Stacker. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. control. Lactate Dehydrogenase (LDH) Release. 340 nm / 70 nm. 4. Near bottom. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 190-1000 nm. Call 1-800-745-2710. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. MMP‑1 inhibition assay. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 20. 001/min at 425 nm. Fully tested with Warranty. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. ) at 490 nm. In vivo anti-malarial assay in mice. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. Details. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Molecular Devices. 00 USD. ). , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. , Sunnyvale, CA). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Molecular Devices SPECTRAmax. Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax Microplate Readers. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Western blot. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Nitric Oxide Assay. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Wells were washed three times. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 313 reference gain check fail. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Our absorbance plate readers feature our PathCheck Sensor technology and. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 305 reference level saturation. Description 1. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). 00 USD. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Subsequently, the mixture was heated at 100 °C for 10. 2. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . SpectraMax ® Plus 384 optics. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Driessen*,MaaikeA. 304 signal level saturation. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Figure 1. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Supplier: Molecular Devices. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 6. Softmax PRO v5. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 5 - 2. albicans pre. 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 0200-7027. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). The reaction was stopped with 1 M hydrochloric acid after 15 min. Molecular Devices. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Emission. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Manager: Yinghua Chen, PhD Contact Email: yinghua. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. One plate carrier adapter is provided with the instrument. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. 5. g. Data System. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. SpectraMax Gemini EM & FLEXstation (bottom read) 1. 5 stars - 1602 reviewsThis assay typically develops in 1. Description 1. SpectraMax Multi-Mode Microplate Readers. Data are expressed as percent of maximum binding for each protein. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Not. The assays contained 0. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Wavelength Selection. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). If depot service is the best option for you we will assist you with logistics to help you keep. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. VersaMax reads 96-well and strip-well plates. Values were determined according to a standard curve (log/log curve-fit). 1 0 100 80 60 40 20 0 4. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. 0% and ± 0. 5. , Fremont, CA, USA). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. StakMax. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). Integration delay. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 5. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. They are for reference only. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. 30. Data were expressed as the mean percent viable cell vs. 8. 6 g sodium acetate trihydrate and 2. 303 unable to cal dark current. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Tunable for filterless flexibility. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Microplate Readers. ScanLater Western Blot Detection Cartridge. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Samples were plated in duplicate (r=0. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMAX 340. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 10. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Acceptor: 665/10 nm. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 340/70 nm. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. 5. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 6) and 0. One plate carrier adapter is provided with the instrument. #ABS PLUS) Methods. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Pharmacokinetic and pharmacodynamic analysis. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. 0, 20 mmol L –1cal-cium chloride). 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. gp70 blocking. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. These models can no longer be repaired. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 1 mL of culture media with 10% FBS. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). These robust readers have been placed in labs from Antarctica to the. Also for: Spectramax 190, Spectramax 340pc384, Versamax. A. ), which was proportional to the number of damaged cells. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. 25–0. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 M DEEMM were mixed in a microtube,. Details. The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Thin layer chromatography (TLC) was. 5 mL of 1 M acetate buffer (pH 4. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. The plate was covered and incubated for one hour at room temperature. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Molecular Devices Corporation 1311. Samples were analyzed in triplicates. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Heme was preincubated for 5 min with A when added before the metals. Immunofluorescence and Aggregation Analysis. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). Crowder, Ziqian Zeng, Alissa N. , Fremont, CA, USA). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. D. ac. 303 unable to cal dark current. SpectraMax Multi-Mode Microplate Readers. (7. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. One plate carrier adapter is provided with the instrument. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Call 1-800-745-2710. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 1 mL of culture media with 10% FBS. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). In this study we used tetanus toxoid (TT) as a well-defined antigen to. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. The values were corrected for nonspecific background by subtracting the OD 650. Microplate Readers. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 024). Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. reader (Molecular Devi ces). For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). California Lightworks uses only the highest quality LEDs from Osram. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 4. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Molecular Devices Spectramax 340PC instructions. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. SPECTRAMAX 340. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. . 0 1. Or scan up to 384 wells at any time to determine the best assay conditions. Buffer (100 mmol L –1acetate buffer, pH 5. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Cell permeabilization. Filterless readers provide consistent results with steady temperature regulation. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Molecular Devices SpectraMax Gemini XS Microplate Reader. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Data were expressed as the mean percent viable cell vs. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The adapter is. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 4) was followed according to Vanacker et al. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. The sensitivities of the hormones are Prolactin: 0. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Other technologies such as imaging. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 0. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). View the Molecular Devices website. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 2. edu Contact Phone: 216-368-8962. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. 2 Chemical Reagents 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. SpectraMax Multi-Mode Microplate Readers. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Call 1-800-745-2710. When necessary, samples were. TDP-43 stress granular cells were treated with 70% ice-cold. Assay buffer was 50 m M Hepes, pH 7. The metals were preincubated with A for 5 min when added before heme. 3 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Read one sample or up to 384. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. 190–850 nm <± 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Wound healing assay. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. well, and strip-well microplates. 1 mL of acetylacetone. Introduction. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. The Catalina. Microplate Reader Shelf. Or scan up to 384 wells at any time to determine the best assay conditions. The reaction mixture was incubated for 5 min. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). . #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 10× trypsin 0. View the Molecular Devices website. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. 316 grating motor driver faulty. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 1 mM MnCl2 and 25 mM CHES buffer, pH 9.